CAS No.:62886-59-3,CM Sephadex C-25
CAS No.：62886-59-3
Synonyms:Carboxymethyl Sephadex C-25; Sephadex C 25
Description
Technical Parameters
CM Sephadex C-25
CAS No.：62886-59-3
Synonyms
Carboxymethyl Sephadex C-25; Carboxymethyl (CM) dextran gel; Sephadex C 50; Sephadex CM C-50; CAS No.：62886-59-3
dextran backbone group
-O-CH₂-COOH
Products Description
|
Item |
Specification |
|---|---|
|
Appearance |
White microspheres, dry |
|
Gel Type |
Ion exchange medium, weak acid type |
|
Structural Component |
Cross-linked dextran |
|
Particle Size |
40–120 μm |
|
Working pH Range |
6–10 |
|
Operating Temperature |
4–40 °C |
|
Total Ion Exchange Capacity |
4–5 mmol/g dry gel |
Storage
Room Temperature
Package
25g/bottle,50g/bottle,100g/bottle,500g/bottle
Service
Customized drum/barrel/bottle packaging acceptable.
Application
It is used for the purification of low-molecular-weight proteins, peptides, nucleotides, macromolecules, etc. by means of ion exchange.
FAQ
Q1:Applications of CM Sephadex C-25
A1:1.Purification of small molecular proteins, peptides and low-molecular-weight enzymes.
2.Suitable for ion exchange separation of biomolecules with molecular weight below 30 kDa.
3.Stable bed volume under varying pH and ionic strength, ideal for routine chromatography and process scale-up.
4.Widely used in laboratory research, sample pre-purification and academic chromatography teaching.
Q2:What are the differences between CM Sephadex C-25 and CM Sephadex C-50?
A2:
| Property | CM Sephadex C-25 | CM Sephadex C-50 |
| Swelling Degree | Low (approx. 5 g water / g dry resin) | High (approx. 15 g water / g dry resin) |
| Suitable Molecules | Small proteins / peptides (< 30 kDa) | Medium-to-large proteins (30–200 kDa) |
| Bed Volume Variation | Low | High (with changes in pH / ionic strength) |
| Flow Rate | Faster | Slower |
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