CAS No.:83382-89-2,QAE Sephadex A-50
CAS No.：83382-89-2
Synonyms：Sephadex QAEA-50;QAE SEPHADEX
Description
Technical Parameters
QAE Sephadex A-50
CAS No.：83382-89-2
Synonyms
Sephadex QAEA-50;QAE SEPHADEX;CAS No.：83382-89-2
dextran backbone group
Products Description
CAS No.：83382-89-2
| Item | Specification |
|---|---|
| Appearance | White microspheres, dry |
| Gel Type | Ion exchange medium, strong base type |
| Structural Component | Cross-linked dextran |
| Particle Size | 40–120 μm |
| Working pH Range | 2–13 |
| Operating Temperature | 4–40 °C |
| Total Ion Exchange Capacity | 2.6–3.4 mmol/g dry gel |
Storage temp.
Room temperature
Application
QAE Sephadex A-50,CAS No.:9041-37-6
1.Application (mainly for 30–100 kDa medium-molecular-weight molecules):Purification of proteins with molecular weight of 30–100 kDa.
2.Separation of macromolecular complexes/fragments:protein aggregates over 100 kDa, viral particles, large nucleic acid fragments (e.g., crude plasmid extraction), antibody fragments (Fab / F(ab')₂).
Package
CAS No.:9041-37-6
100g/bottle,500g/bottle,1000g/bottle,
Service
Customized drum/barrel/bottle packaging acceptable.
FAQ
Q: How to store used QAE Sephadex A-50 ?
A: 1.Used resin shall be stored sealed at 4 ℃ in well-ventilated, dry and clean place, with 20% ethanol as storage solution. Do not freeze.
2.Used chromatography columns shall be stored at 4 ℃ in 20% ethanol solution.
3.Unused product should be kept tightly sealed at room temperature.
Q: How to choose between QAE Sephadex A-25 and QAE Sephadex A-50?
A: 1.If the sample molecular weight ≤ 30 kDa (polypeptides, oligonucleotides, small molecular proteins), choose A-25 (small pore, high charge density, cost-effective).
2.If the sample molecular weight is 30–100 kDa (medium-sized proteins, enzymes, antibody fragments), choose A-50 (large pore, high binding capacity, suitable for process scale-up).
3.For samples > 100 kDa (macromolecules, viruses, plasmids), A-50 is preferred (mainly adsorbed on the particle surface with acceptable capacity).
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