Sephadex LH-20

  Sephadex LH-20

    CAS No.:9041-37-6

 

 

    LIPOPHILIC SEPHADEX CAS 9041-37-6; LIPOPHILIC SEPHADEX;CAS No.:9041-37-6

 

 

  CAS No.:9041-37-6

 

 

 

 CAS No.:9041-37-6

Item	Specification
Appearance	White microspheres, porous
Gel Type	Gel filtration medium, lipophilic type
Structural Component	Cross-linked dextran
Particle Size	100–200 mesh
operating pH range	2–13
Operating Temperature	4–40 °C
Exclusion Limit	4000–5000 (depending on solvent)
Formular	C9H8N2O3S
Molecular weight	224.24
HS Code	29400090

 

 

    Room Temperature

 

   CAS No.:9041-37-6

   Taking advantage of molecular sieve effect, it is suitable for the separation of lipophilic molecules in organic solvents, allowing highly economical large-scale preparation of various natural products, such as steroids, terpenoids, lipids, small-molecule peptides, etc.

 

    CAS No.:9041-37-6

    100g/bottle,1kg/drum,5kg/drum,25kg/drum.

 

 

   Customized drum/barrel/bottle packaging acceptable.

 

  FAQ about CAS No.:9041-37-6

 

Q1: What is the matrix of Sephadex LH-20?

  A1: Hydroxypropyl dextran

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Q2: What are the main solvent systems for Sephadex LH-20?

  A2: Water, methanol, ethanol, chloroform, ethyl acetate, etc.

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Q3: What are the differences between Sephadex LH-20, regular G-type and ion-exchange type Sephadex?

  A3:Regular G-type (G-25/G-50): Hydrophilic only, compatible with aqueous phase exclusively; cannot be used in pure organic solvents.

Ion-exchange type (SP-C25/QAE-A25): Charged + hydrophilic, designed for aqueous phase ion exchange.

LH-20: Lipophilic + hydrophilic, compatible with both aqueous and organic phases; primarily functions as a molecular sieve, with additional partition/adsorption effects.

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  Q4: What are the main applications of Sephadex LH-20?

  A4:High-resolution separation of small-molecule natural products, homologs and diastereomers.

                                                  

  Q5: Core Performance Comparison: Sephadex LH-60 vs LH-20?

 

Core Dimension	Sephadex LH-20	Sephadex LH-60	Direct Impact on Application
Matrix Skeleton	Based on Sephadex G-20 matrix, with high crosslinking degree and extremely small pore size	Based on Sephadex G-60 matrix, with low crosslinking degree and large pore size	Directly determines the molecular size that can enter the gel, which is the core source of application differences
Water Absorption of Dry Gel	2.0 mL/g	6.0 mL/g	The higher the water absorption, the larger the volume after swelling, the larger the pore size, and the larger the molecules that can be accommodated
Molecular Sieve Separation Range (Globulin Calibrated)	100-4000 Da	400-10000 Da (Aqueous Phase) / 400-20000 Da (Organic Phase)	LH-20 has an upper limit of only 4000 Da, specializing in small molecules; LH-60 has an upper limit of up to 20000 Da, covering the full range of macromolecules
Separation Resolution	Extremely high separation resolution for small molecules in the range of 100-4000 Da	Good adaptability to samples with a wide molecular weight range, with slightly lower resolution for small molecules than LH-20	LH-20 is suitable for the fine separation of isomers and homologs; LH-60 is suitable for the rapid fractional separation of different molecular weight segments
Industrial Applicability	Slightly high back pressure, with a maximum flow rate of 300 cm/h	Low back pressure, with a maximum flow rate of 400 cm/h, and better gel rigidity	LH-60 is more suitable for large-scale scale-up production with large-diameter industrial chromatography columns

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