CAS No.:52219-08-6,QAE Sephadex A-25
CAS No.：52219-08-6
Synonyms：Sephadex QAEA-25;QAE SEPHADEX
Description
Technical Parameters
QAE Sephadex A-25
CAS No.：52219-08-6
Synonyms
Sephadex QAEA-25; QAE SEPHADEX; CAS No.：52219-08-6
dextran backbone group
－N＋(CH2CH3)3Cl－
Products Description
| Item | Specification |
|---|---|
| Appearance | White microspheres, dry |
| Gel Type | Ion exchange medium, strong base type |
| Structural Component | Cross-linked dextran |
| Particle Size | 40–100 μm |
| Working pH Range | 2–3 |
| Operating Temperature | 4–40 °C |
| Total Ion Exchange Capacity | 2.6–3.4 mmol/g dry gel |
Storage temp.
Room temperature
Application
Application (mainly for ≤30 kDa small-molecular-weight molecules):
1.Purification of small proteins and polypeptides:proteins and polypeptides with molecular weight ≤30 kDa (e.g., Cytochrome C, Insulin, short peptides).
2.Separation of nucleic acids and derivatives:oligonucleotides, short-chain DNA/RNA, nucleotides, coenzymes (e.g., ATP, NAD⁺).
3.Purification of polysaccharides and oligosaccharides:acidic polysaccharides and oligosaccharides (e.g., heparin fragments, sodium alginate oligosaccharides).
Package
CAS No.:52219-08-6
100g/bottle,500g/bottle,1000g/bottle,
Service
Customized drum/barrel/bottle packaging acceptable.
FAQ
General precautions for QAE Sephadex A-25 and QAE Sephadex A-50.
1.Equilibration / Elution buffer: pH 2–10. Binding under low salt condition (20–50 mM); elution under high salt condition (0.1–1 M NaCl).
2.Elution mode: linear salt gradient and step elution are applicable; pH gradient is also allowed (avoid extreme pH conditions).
3.Cleaning and regeneration: rinse sequentially with 2 M NaCl, 0.1 M NaOH, purified water or buffer; the resin can be reused after regeneration.
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